Formation of gutingimycin: analytical investigation of trioxacarcin A-mediated alkylation of dsDNA

نویسندگان

  • Ansgar Fitzner
  • Holm Frauendorf
  • Hartmut Laatsch
  • Ulf Diederichsen
چکیده

Formation and fragmentation of recognition complexes between trioxacarcin A and various DNA sequences were examined by temperature-dependent UV and CD spectroscopy, HPLC analysis, and ESI mass spectrometry with regard to reaction conditions, intermediates, products, mechanism, and sequence specificity. Cleavage of the trioxacarcin-DNA complexes provided the natural product gutingimycin by guanine abstraction. The resulting DNA with an abasic site was further cleaved into a DNA fragment with a furanyl unit at the 3'-end and an oligonucleotide with a phosphorylated 5'-end.

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عنوان ژورنال:
  • Analytical and Bioanalytical Chemistry

دوره 390  شماره 

صفحات  -

تاریخ انتشار 2008